Journal: Neuron

Article Title: A Visual-Cue-Dependent Memory Circuit for Place Navigation

doi: 10.1016/j.neuron.2018.05.021

Figure Lengend Snippet:

Article Snippet: During all of the training and test trials, videos were recorded and the animal’s locations were tracked using a custom-written MATLAB program.

Techniques: Virus, Recombinant, Software

Journal: bioRxiv

Article Title: Variant-to-gene-mapping followed by cross-species genetic screening identifies GPI-anchor biosynthesis as novel regulator of sleep

doi: 10.1101/2021.12.19.472248

Figure Lengend Snippet: a , Design of orthologous gene screen. b , Total sleep minutes over a 24-h period in viable RNAi crosses (73 lines, n > 16 per line). Dotted lines and greyed area indicate two std. dev from the mean for every animal tested in either direction. Blue dots indicate control sleep responses, while red dots indicate sleep responses of RNAi lines that fall outside two std. dev. c , Sleep profiles of two independent RNAi lines targeting PIG-Q ( PIG-Q -RNAi : red; PIG-Q -RNAi : blue). d , Knockdown of PIG-Q in significantly increases total sleep (t-test, PIG-Q -RNAi t 260 = 11.42; P <0.0001; PIG-Q -RNAi t 184 = 4.282; P = <0.0001). e , Knockdown of PIG-Q in significantly decreases waking activity (t-test, PIG-Q -RNAi t 260 = 11.45; P = <0.0001; PIG-Q -RNAi t 184 = 11.09; P <0.0001). ( f-h ), The Drosophila ARousal Tracking (DART) system records fly movement while simultaneously controlling periodic mechanical stimuli. f , The DART system was used to measure arousal threshold and reactivity. Arousal threshold was measured on sleeping flies using mechanical stimuli of increasing strength. Reactivity was measured by assessing the proportion of flies that react to a single mechanical stimulus for each bin of immobility. g , Knockdown of PIG-Q significantly increases arousal threshold (REML, PIG-Q -RNAi : F 1,73 = 4.267, P =0.0424; PIG-Q -RNAi : F 1,102 = 16.42, P <0.0001). This occurs during the day for both independent RNAi lines ( PIG-Q -RNAi P =0.0127; PIG-Q -RNAi : P =0.0002), while an increase in arousal threshold only occurred in one line during the night ( PIG-Q -RNAi : P =0.4308; PIG-Q -RNAi : P =0.0020). h , Knockdown of PIG-Q significantly decreases nighttime reactivity (ANCOVA with bout length as covariate, PIG-Q -RNAi : F 1,661 = 107.1, P <0.0001; PIG-Q -RNAi : F 1,594 = 24.87, P <0.0001). For sleep profiles, error bars represent +/-standard error from the mean. For violin plots, the median (solid black line) is shown. White background indicates daytime, while gray background indicates nighttime. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Article Snippet: The vibrational stimulus, video tracking parameters, and data analysis were performed using the DART interface developed in MATLAB (MathWorks, Natick, Massachusetts).

Techniques: Activity Assay